Illumina Announces Moleculo Long Read Technology and Phasing As Service

Illumina Announces Moleculo Long Read Technology and Phasing As Service


Illumina kept its promise of making Moleculo’s Long Read Technoogy as service in 2013.  Illumina announced today that Illumina’s FastTrack Services will be offering Long-Read Sequencing Services and new Phasing Analysis. With the two new additional services, Illumina can provide whole-genome results within twelve weeks. Illumina’s Long Read sequencing service is not only available for human, but for any organism with genome size bigger than 100Mb.

Illumina’s Long Read Sequencing technology needs 1 µg of genomic DNA and can produce fragments of size up to 10 kilobases. A single library produces about 600Mb of long read sequence data. Announcing the long read sequencing as service, Illumina also said that the Long Read sequencing technology will be soon available as a kit.

If you are interested in learning more about the Long Read sequencing technology, check out the first publication from Stephen Quake’s group describing Moleculo Long Read Sequencing technology as LRSeq and applying to sequence the genome of a chordate, The genome sequence of the colonial chordate, Botryllus schlosseri.  llumina has also made available some long read sequence data from Fly genome in its Basespace.


Illumina Moleculo Technology


Moleculo, acquired by Illumina in late 2012, has developed an innovative technology for generating long reads that combines a new sample prep method and genome analysis tools. The technology breaks DNA into large fragments that are sequenced on standard Illumina sequencing systems for subsequent assembly into synthetic long reads using proprietary informatics.
The Moleculo sample prep is a full end-to-end workflow from DNA to sequencing-ready library. Applications for this long read technology include de novo assembly (of human, plant or other complex genomes), human genome phasing, and cancer sequencing.

Reconstructs fragments into long read data

Moleculo technology begins by fragmenting genomic DNA to approximately 10 kilobases. Next, these fragments are clonally amplified, sheared, and marked with a unique barcode. They are then sequenced with Illumina technology. The short sequence reads originating from each molecule are assembled separately. The end result is a full sequence of all the fragments. Essentially, short‐read data is reconstructed into long reads.

Increased throughput, read lengths, and speed

Moleculo technology has the potential to improve the efficiency and accuracy of many existing DNA sequencing applications while increasing the coverage of clinically important genes. These advantages allow for the phased re‐sequencing of human genomes and rapid de novo sequencing of plant and animal genomes.

Sample prep for the Moleculo technology currently takes about 2 days to complete, not including sequencing time. It takes less than 4 days to phase a human genome on the HiSeq 2500, going from sample to answer.

Better genome phasing and de novo sequencing

The long reads produced typically span more than one heterozygous SNP. This allows Moleculo’s proprietary phasing algorithm to “stitch” multiple long reads into a single haplotype and thus phase the genome. The Moleculo technology simplifies de novo sequencing because large repeat regions in the DNA fragments can easily be spanned


Related Papers

  1. The genome sequence of the colonial chordate, Botryllus schlosseri

Related Posts

  1. Illumina Acquires Moleculo Inc. for Longer Reads.
  2. Moleculo Man: Mickey Kertesz on Illumina’s Sub-Assembly Acquisition.
  3. Moleculo data presented at Illumina Scientific Summit.
  4. First Data Set from FastTrack Long Reads Early Access Service.
  5. Long-Read Sequencing Service FAQ.

1 comment to Illumina Announces Moleculo Long Read Technology and Phasing As Service

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