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SortmeRNA: 快速准确筛选NGS序列集合中的rRNA序列

标题:

SortMeRNA: fast and accurate filtering of ribosomal RNAs in metatranscriptomic data

摘要:

Motivation: The application of next-generation sequencing (NGS) technologies to RNAs directly extracted from a community of organisms yields a mixture of fragments characterizing both coding and non-coding types of RNAs. The task to distinguish among these and to further categorize the families […]

ProDeGe: 自动去除基因组序列中的污染序列

文章标题:

ProDeGe: a computational protocol for fully automated decontamination of genomes

文章摘要:

Single amplified genomes and genomes assembled from metagenomes have enabled the exploration of uncultured microorganisms at an unprecedented scale. However, both these types of products are plagued by contamination. Since these genomes are now being generated in a high-throughput manner and sequences from […]

Insights into the phylogeny and coding potential of microbial dark matter

Insights into the phylogeny and coding potential of microbial dark matter

Supplementary Information

SAG assembly

The draft genome of all but 13 SAGs was generated at the DOE Joint genome Institute (JGI) using the Illumina technology. An Illumina standard shotgun library was constructed and sequenced using the Illumina HiSeq 2000 platform. All general […]